A scientific seminar will be held at the Institute of Biophysics
On October 31, 2025, at 10:00 a.m., a scientific seminar dedicated to the 120th anniversary of academician Yusif Mammadaliyev will be held at the Institute of Biophysics of the Ministry of Science and Education of the Republic of Azerbaijan.
The seminar will cover the following topics:
- “The role of cytochrome C as a target for cancer prognosis and therapy”
- “Kinetics of cytochrome C-driven ABTS oxidation reaction”
Presentations will be delivered by Sevda Mahmudova and Fidan Qudratova, junior researchers of the Molecular and Cellular Biochemistry Laboratory.
You are kindly invited to attend the seminar.
Brief information:
1. The role of the cytochrome C as a target for cancer prognosis and therapy
Cytochrome c (cyt c) functions as an electron carrier in the mitochondrial respiratory chain and plays a key role in apoptosis. When released into the cytoplasm, it activates the intrinsic apoptotic pathway and can enter the bloodstream. Changes in serum cyt c levels reflect inhibition or induction of apoptosis, making it a valuable biomarker for cancer prognosis and monitoring chemotherapy response. Various sensitive biosensors have been developed to quantify cyt c in serum. Additionally, delivering exogenous cyt c to cancer cells induces apoptosis, and several protein- and nanoparticle-based delivery systems have been designed for this purpose. Overall, cytochrome c holds great potential as both a diagnostic and therapeutic target in cancer. Our experiments show that the ABTS oxidation reaction can be an important model for the functional characterization of cytochrome.
2. Kinetics of cytochrome C-driven ABTS oxidation reaction
As is well known, cytochrome c is a key electron carrier in cellular metabolism; however, how this function changes under different cellular conditions — particularly during pathological processes — remains not fully understood. In this study, we investigated the electron transfer properties of cytochrome c through the ABTS oxidation process. Using the Lineweaver–Burk kinetic model, we analyzed the reaction of cytochrome c with ABTS and H₂O₂ substrates. Our results indicate that the ABTS oxidation reaction can serve as a simple yet reliable biocatalytic model for studying the electron transfer capacity of cytochrome c.
